Can IVF affect NIPT results?

IVF itself does not make NIPT inaccurate, but there are a few IVF-specific considerations worth knowing. Vanishing twin. Multiple embryo transfer, and multiple pregnancy generally, is more common after IVF. If one twin stops developing early, its DNA can persist in your blood and produce a false positive result. Tell whoever orders your NIPT if you had a multiple pregnancy at any stage, even briefly — this is easily forgotten and it changes interpretation. Twins. In twin pregnancies, inconclusive results are more common. Laboratories generally use the lower of the two fetal fractions to judge sample adequacy, which makes a no-call more likely. Donor eggs or surrogacy. NIPT analyses DNA from the pregnancy alongside DNA from the person carrying it. Where a donor egg or a surrogate is involved, the laboratory needs to know, because the interpretation differs. Tell them upfront rather than assuming it is on the referral. After PGT-A. Having had PGT-A does not remove the value of NIPT, and neither test is diagnostic — both analyse placental-lineage material, so both share the same limitation. ESHRE recommends prenatal diagnosis is offered to all women who become pregnant following PGT. Fetal fraction is not affected by IVF as such, though it is lower with higher BMI and at earlier gestations. The practical advice: make sure the laboratory knows your full pregnancy history, including how conception occurred and whether there was ever a second sac.